Abstract
5'-O-[N-(L-glutamyl)-sulfamoyl] adenosine is a potent competitive inhibitor of E. coli glutamyl-tRNA synthetase with respect to glutamic acid (K(i) = 2.8 nM) and is the best inhibitor of this enzyme. It is a weaker inhibitor of mammalian glutamyl-tRNA synthetase (K(i) = 70 nM). The corresponding 5'-O-[N-(L-pyroglutamyl)-sulfamoyl] adenosine is a weak inhibitor (K(i) = 15 microM) of the E. coli enzyme.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine / analogs & derivatives*
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Adenosine / chemical synthesis
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Adenosine / pharmacology
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Animals
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Binding Sites
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Binding, Competitive
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Enzyme Inhibitors / chemical synthesis*
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Enzyme Inhibitors / pharmacology*
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Escherichia coli / enzymology*
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Glutamate-tRNA Ligase / antagonists & inhibitors*
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Glutamate-tRNA Ligase / metabolism
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Liver / enzymology
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Mice
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RNA, Transfer, Glu / metabolism
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Substrate Specificity
Substances
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Enzyme Inhibitors
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RNA, Transfer, Glu
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Glutamate-tRNA Ligase
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Adenosine